Volume-2 ,Issue-1, January-2026

Thiocolchicoside, a muscle relaxant with limited aqueous solubility, was formulated into nano lipid carriers (NLCs) to enhance solubility, stability, and controlled drug delivery. Preliminary physicochemical characterization confirmed the drug’s purity, identity, and suitability for formulation, with a melting point of 191 °C, pH of 7.1, and λmax at 376 nm. NLCs were prepared using heat homogenization and sonication, employing stearic acid, oleic acid, Tween 80, and sodium lauryl sulfate. Five formulations (F1–F5) were evaluated for particle size, polydispersity index, zeta potential, entrapment efficiency, morphology, and in vitro drug release. Formulation F1 exhibited the most favorable characteristics, including the smallest particle size (42.53 nm), narrow polydispersity index (27.4%), zeta potential of –13.8 mV, and highest entrapment efficiency (94.56%). SEM analysis confirmed spherical, homogeneous Nanoparticles. In vitro studies demonstrated sustained drug release up to 16 hours, with F1 achieving 93.43% cumulative release, indicating controlled and efficient drug delivery. These findings suggest that thiocolchicoside-loaded NLCs, particularly F1, offer a promising strategy for improving bioavailability, therapeutic efficacy, and patient compliance, supporting their potential for future in vivo and clinical applications.

Keyword: Thiocolchicoside, Nano lipid carriers (NLCs), Pre-formulation study, Entrapment efficiency, Particle size